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KMID : 0369319890090030325
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Allergy
1989 Volume.9 No. 3 p.325 ~ p.334
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Glucocorticoid Hormones Decrease the Proliferation and Viability of Cultured Mast Cells
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Kyung-Up Min
John J. Costa
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Abstract
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The local administration of glucocorticoid hormones decreases tissue mast cell numbers. To explore the direct effect of steroids on mast cells, we cultured the interleukin 3 dependent murine mast cell line, PT-18, in interleukin-3 containing WEHI-3 conditioned medium in the presence or absence of dexamethasone. Viable cell counts, and tritiated thymidine, uridine and leucine incorporation were compared in the daxamethasone treated and control mast cell populations. Both cell viability and the uiable cell count decreased in a dose dependent manner. At low concentration of dexamethasone, cell viability was maintained while cell proliferation was inhibited. Exposure of mast cells to dexamethasone (10 M for 24hrs) cause inhibition of thymidine, uridine and leucine incorporation to 9,5.2":, 78.3/p and 82.2% of control values respectively. This effect could not be reuersed by increasing the concentration of IL-3 in the culture medium. The effects of dexamethasone on rat basophilic leukemia cells, HL-60 cells and rat embryo dermal fibroblasts were also examined. Inhibition of thymidine incorporation was limited to the rat basophilic leukemia cells. We conclude that dexamethasone acts as a growth inhibiting and ultimately, cytotoxic agent on mast cells.
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